Sisebenzisa iikuki ukuphucula amava akho.Ngokuqhubeka nokukhangela le webhusayithi, uyavumelana nokusetyenziswa kwethu kweekuki.Iinkcukacha ezithe xaxe.
Kukho imfuneko engxamisekileyo yokuba iingxelo zophando lwebhayoloji yeeseli ezanyisayo zibe zisemgangathweni ngakumbi kwaye zibe neenkcukacha, kunye nokulawula ngcono kunye nokulinganisa iimeko zokusingqongileyo zenkcubeko yeseli.Oku kuya kwenza imodeli yefiziyoloji yomntu ichaneke ngakumbi kwaye ibe negalelo ekuphindaphindeni kophando.
Iqela lezazinzulu ze-KAUST kunye noogxa baseSaudi Arabia nase-United States bahlalutya amaphepha angama-810 akhethwe ngokungaqhelekanga kwimigca yeseli ye-mammalian.Bangaphantsi kwama-700 kubo ababandakanyekayo kwi-1,749 yovavanyo lwenkcubeko yeseli yomntu ngamnye, kubandakanywa idatha efanelekileyo malunga neemeko zokusingqongileyo zendlela yenkcubeko yeseli.Uhlalutyo lweqela lubonisa ukuba umsebenzi omninzi kufuneka wenziwe ukuphucula ukufaneleka kunye nokuveliswa kwakhona kwezifundo ezinjalo.
Hlakulela iiseli kwi-incubator elawulwayo ngokwemigaqo eqhelekileyo.Kodwa iiseli ziya kukhula kwaye "ziphefumle" ekuhambeni kwexesha, zitshintshisa igesi kunye nendalo engqongileyo.Oku kuya kuchaphazela imeko yendawo apho zikhula khona, kwaye kunokutshintsha iasidi yenkcubeko, ioksijini enyibilikileyo, kunye neparamitha yecarbon dioxide.Olu tshintsho luchaphazela ukusebenza kweeseli kwaye lunokwenza imeko yomzimba yahluke kwimeko esemzimbeni womntu ophilayo.
"Uphando lwethu lugxininisa umlinganiselo apho izazinzulu zingawunaki ukubeka iliso kunye nokulawula indawo yeselula, kwaye ukuya kuthi ga apho iingxelo zibenza bakwazi ukufikelela kwizigqibo zesayensi ngeendlela ezithile," kusho uKlein.
Ngokomzekelo, abaphandi bafumanisa ukuba malunga nesiqingatha samaphepha ohlalutyo awazange achaze ubushushu kunye ne-carbon dioxide setting yenkcubeko yeeseli zabo.Ngaphantsi kwe-10% ibike umxholo we-oxygen emoyeni kwi-incubator, kwaye ngaphantsi kwe-0.01% ibike i-acidity ye-medium.Akukho maphepha achazwe ngeoksijini enyibilikileyo okanye icarbon dioxide kumajelo eendaba.
Simangalisa kakhulu ukuba abaphandi baye bangayinaki kakhulu imiba yokusingqongileyo egcina amanqanaba afanelekileyo e-physiologically ngexesha lenkqubo yonke yenkcubeko yeeseli, ezifana ne-acidity yenkcubeko, nangona iyaziwa ukuba oku kubalulekile ekusebenzeni kweseli.”
Iqela likhokelwa nguCarlos Duarte, i-ecologist yaselwandle e-KAUST, kunye no-Mo Li, i-stem cell biologist, ngokubambisana noJuan Carlos Izpisua Belmonte, i-biologist yophuhliso kwi-Salk Institute.Okwangoku unguprofesa otyelele e-KAUST kwaye ucebisa ukuba izazinzulu ze-biomedical ziphuhlise iingxelo ezisemgangathweni kunye neenkqubo zokulawula kunye nokulinganisa, ngaphezu kokusebenzisa izixhobo ezikhethekileyo zokulawula indawo yenkcubeko yeeseli ezahlukeneyo.Iijenali zenzululwazi kufuneka ziseke imigangatho yokunika ingxelo kwaye zifuna ukubekwa iliso okwaneleyo kunye nolawulo lwe-asidi yemidiya, ioksijini enyibilikisiweyo kunye nekhabhon diokside.
"Ukunika ingxelo engcono, ukulinganisa, kunye nokulawula iimeko zokusingqongileyo zenkcubeko yeeseli kufuneka ziphucule amandla oososayensi ukuphinda kunye nokuvelisa iziphumo zovavanyo," kusho u-Alalslami."Ukujongwa ngokusondeleyo kunokuqhuba izinto ezintsha ezifunyenweyo kunye nokwandisa ukubaluleka kophando lwangaphambili kumzimba womntu."
Isazinzulu saselwandle uShannon Klein siyacacisa: “Inkcubeko yeeseli ezincancisayo sisiseko sokwenziwa kwezitofu zokugonya iintsholongwane kunye nobunye ubugcisa bebhayoloji."Ngaphambi kovavanyo kwizilwanyana nakubantu, zisetyenziselwa ukufunda ibhayoloji esisiseko yeeseli, ukuphindaphinda iindlela zesifo, kunye nokufunda ubutyhefu bamachiza amatsha."
Klein, SG, njl. (2021) Ukungahoywa ngokubanzi kolawulo lokusingqongileyo kwinkcubeko yeseli yezilwanyana ezincancisayo kufuna iindlela ezifanelekileyo.Ubunjineli beNdalo yeBiomedical.doi.org/10.1038/s41551-021-00775-0.
iithegi: B iseli, iseli, inkcubeko yeseli, incubator, iseli mammalian, ukuvelisa, ioksijini, pH, physiology, preclinical, uphando, T cell
Kolu dliwano-ndlebe, uNjingalwazi uJohn Rossen wathetha ngokulandelelana kwesizukulwana esilandelayo kunye nefuthe laso ekuxilongeni isifo.
Kolu dliwano-ndlebe, i-News-Medical ithethe noNjingalwazi uDana Crawford malunga nomsebenzi wakhe wophando ngexesha lobhubhani we-COVID-19.
Kulo dliwano-ndlebe, i-News-Medical yathetha noDkt. Neeraj Narula malunga nokutya okucutshungulwayo kunye nokuba oku kunokunyusa njani umngcipheko wakho wesifo sofuba (IBD).
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Ixesha lokuposa: Sep-07-2021
